Analysis of Chlorophyll Content in Rice Leaves by Chlorophyll Content Analyzer

The important energy of the crop comes from its own leaves, so the function and yield of the leaves are closely related, especially the health of the leaves during rice planting has a great impact. If the leaf premature aging will shorten its photosynthesis time, causing the grain is not full, resulting in reduced production. What is the reason for the accelerated aging of the leaves and the phenomenon of premature aging? Strong light is one of the important factors that cause aging. Chlorophyll content analyzer can effectively determine the chlorophyll content of the leaves without damaging the leaves.

Under natural strong light, excess light energy causes the accumulation of reactive oxygen species, hydrogen peroxide, and membrane lipid peroxidation product (MDA), resulting in chlorophyll decay and photooxidative premature aging. Some scholars believe that the photosynthetic rate of materials with low total chlorophyll content is lower than that of materials with high chlorophyll content, which can not fully absorb and transfer strong light, and has poor tolerance to strong light.

The trial was conducted in Jingzhou, Hubei Province in 2007. The planting was conducted on May 6, 2007 and transplanted on June 8. The planting density was 13.3 cm x 26.7 cm. Thirty plants were planted in each plot, and three replicates were arranged randomly. The SPAD value of the leaf was measured. After the first ear of a single plant was withdrawn, the ear was tagged, and the leaves of the ear of the tag were measured each time thereafter. Three populations were observed in each plot in test group 1. The leaf SPAD values ​​were recorded on the ninth day after the beginning of the first plant, measured once every four days, and measured continuously for five times. The experimental group 2 was measured from the ninth day after the first ear. Five cells were observed in each plot, measured once every 5 days, and measured continuously three times. The leaf chlorophyll content analyzer was used to measure the upper, middle and lower parts of the flag leaf, and the average value was taken as the SPAD value of the leaf.

The relationship between the chlorophyll content and the SPAD value was determined by the chlorophyll content analyzer. The low chlorophyll content group had a larger decrease in chlorophyll content within 9-13 days after heading, and the chlorophyll content group was larger, but the results were reversed within 14}18 days after heading. At the early filling stage, the chlorophyll content of the low chlorophyll content group had a large decrease in chlorophyll content, and the chlorophyll content of the low chlorophyll content group had a large decrease in the high chlorophyll content group.

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